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0.1 ml of enzyme extract was added.
The reaction mixture was mixed with 0.3 mL of enzyme solution and incubated for 2 min.
It consisted of 1 mL of enzyme solution, precipitant, glutaraldehyde and an additive.
The reaction mixture consisted of 1 mL of enzyme extract and guaiacol as a substrate.
The reaction mixture contained 0.4 mL of enzyme extract, 0.4 of mL 100 mM acetate buffer (pH 4.8), and 0.2 mL of 0.05 M bovine hemoglobin.
The reaction was initiated by addition of 0.1 mL of enzyme solution (concentration of stocks: MhyADH = 1.5 mg/ml, TADH = 1.0 mg/mL).
Similar(8)
The non-enzymatic hydrolysis rate of SO was determined after incubation at 35 °C, 180 rpm for 1 h in 4 mL of enzyme-free systems, which pH was also measured by pH meter.
The solid to solution ratio of the system was 0.1 gm of lyophilized bone matrix per 25 ml of enzyme-phosphate buffer solution.
Maximal amylase production of 464 units/ml of enzyme was observed in the presence of 100% moisture, 0.1% fructose and 0.01% ammonium sulphate.
The best condition for the concentration of Candida rugosa lipase was at −10 °C and 80% (w/w) acetone saturation, which resulted in 198 U/mL of enzyme activity and 11.7-fold concentration factor (CF).
The maximum decolorization (96.34%) of 7.5 mg/L dye was achieved at 42 U/mL of enzyme dose, 0.25 mM of H2O2 and 0.5 mM of p-coumaric acid.
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