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An MBS solution (6.4 mM) was made by dissolving 2 mg MBS (6.4 μmol) in 1 mL of dimethyl sulphoxide (DMSO).
Intravenous administration of up to 1 ml of dimethyl sulfoxide/kg caused profound hypotension and eventually failure of neuromuscular transmission.
5 mM of p NPL was dissolved in 10 mL of dimethyl sulphoxide (DMSO).
For each sample, 100 mg of crude extract were dissolved in 10 mL of dimethyl sulfoxide (DMSO).
Working standard with 10 mL of dimethyl acetamide and dilution with the mobile phase up to 100 mL.
Nafion solution [5%% (w/w), DuPont] was firstly evaporated at 80 °C and re-dissolved in 10 mL of dimethyl sulfoxide (DMSO).
For the preparation of DBT CNT composite solution, 10 mg of DBT and 10 mg of CNTs were added into the 7.5 mL of dimethyl formamide (DMF).
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The resultant extract was dissolved in 1 ml of dimethyl-sulfoxide (DMSO) (Sigma) and kept at 4°C as stock solution.
Stock samples at 1 mg/mL of dimethyl sulfoxide (DMSO) (Sigma-Aldrich, USA) were prepared and keep at 4 °C.
Antibody bead complex is covalently cross-linked using 10 mg/ml of dimethyl pimelimidate dihydrochloride (DMP; Sigma) for 45 min at room temperature and 5 mg/ml of DMP for 30 min.
Fixed tissues were washed with 2 mM MgCl2, 0.01% Deoxycholate, 0.02% NP-40 in 0.1 M sodium phosphate buffer at 4°C with rocking and stained with X-gal in the wash solution containing 5 mM K4Fe CN 6, 5 mM K3Fe CN 6 and 40 mg/ml of dimethyl formaide) at 37°C with shaking for 3 hours.
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