Sentence examples for ml of digestion from inspiring English sources

Calvaria were digested in 10 mL of digestion solution for 15 min at 37 °C with constant agitation.

The arterial tissues were then minced and digested in 5 ml of digestion solution (0.25 mg/ml soybean trypsin inhibitor, 0.125 mg/ml elastase, 2.0 mg/ml crystallized bovine albumin, 10 mg/ml collagenase I, and 15 mM HEPES) at 37°C for 45 min.

Plugs were digested for 48 h at 55°C in 5 ml of digestion buffer containing 100 m M EDTA (pH 8.0), 0.2% sodium deoxycholate, 1% sodium lauryl sarcosine, and 1 mg ml−1 proteinase K.

Lungs were excised on days 3, 7, and 14 post inoculation and digested enzymatically at 37°C for 30 minutes in 10 ml of digestion buffer (RPMI 1640 and 1 mg/ml of collagenase type IV [Sigma Chemical Co., St . Louis MO.]) with intermittent (every 10 min) stomacher homogenizations.

Lungs were excised at specific time points post- inoculation and digested enzymatically at 37°C for 30 minutes in 10 ml of digestion buffer (RPMI 1640 and 1 mg/ml of collagenase type IV [Sigma-Aldrich, St . Louis MO.]) with intermittent (every 10 min) stomacher homogenizations.

After removing the trypsin solution, the cartilage was digested at 37°C with type II collagenase using 1 ml of digestion solution per 100 mg tissue (Gibco, Grand Island, NY, USA).

Cells were washed with PBS and nuclei prepared by resuspension in 1 ml aliquots of digestion buffer (Tris-HCl 15 mℳ pH7.5, NaCl 15 mℳ, KCl 60 mℳ, MgCl2 5 mℳ, glucose 300 mℳ, EGTA 0.5 mℳ, NP40 0.1%).

Tissue was incubated at 37°C under gentle rotation for 15 min. Undigested tissue was allowed to settle for 5 min; the supernatant fraction was then collected by aspiration, and 25 ml of fresh digestion medium was added to the tissue fraction.

Equal amounts of prepared nuclei were washed and resuspended in 1 ml of nuclear digestion buffer (50 mM Tris-HCl, pH 8.0, 0.1 mM CaCl2).

For the in vitro studies on cells, an aliquot (5 mL) of each digestion solution was freeze dried (freeze-dryer Edwards Modulyo, UK).

Nuclei (5 × 10 cells) were resuspended in 5 mL of MNase digestion buffer (50 mM Tris HCl, 320 mM sucrose, 4 mM MgCl2, 1 mM CaCl2, pH 7.6).