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Finally, 3 mL of diethyl amine were added to the mixture.
For each 1 mL of TFA solution, 10 mL of diethyl ether was used.
Extraction of samples was achieved by vortexing three times with 20 mL of diethyl ether (basic extracts).
The resulting solid was redissolved in 20 ml of diethyl ether and washed with water (2 × 10 ml).
After 24 h, it was cooled down to room temperature, and the reaction mixture was poured into 500 mL of diethyl ether.
The obtained product was coagulated with 200 mL of diethyl ether and then filtered by Omnipore™ membrane with a 0.2-μm pore size.
For the quantitative analysis of α-pinene, α-phellandrene and octylacetate, at 40 °C, two stock solutions Q were prepared using 10 mg of each analytes and dissolving them in 100 mL of diethyl ether.
For the quantitative analysis of α-pinene, γ-terpinene and linalool at 22 °C, three stock solutions K were prepared using 50 mg of each analytes and dissolving them in 100 mL of diethyl ether.
For the quantitative analysis of β-pinene limonene, γ-terpinene, linalool, linalyl acetate, five stock solutions A were prepared using 150 mg of each analytes and dissolving them in 5 mL of diethyl ether.
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We extracted the radioactive hormones and their metabolites by adding 2 times 5 ml of diethyl-ether to 0.2 ml of sample and shaking for 30 min.
Peptides were precipitated and washed three times with 10 ml portions of diethyl ether, dried, dissolved in distilled water and lyophilized.
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