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5 ml of copper acetate solution was added to 5 ml of the extracts.
5 g Lissapol L as surfactant was added to 100 ml of copper nano sol coating solution.
The upper isooctane layer (3.0 mL) containing FFA was transferred into a test tube, and 1.0 mL of copper reagent was added and vortexed.
The copper removal experiments were carried out by stirring 5 mg of TAPP in 100 mL of copper solution at 25°C.
Typically, 0.3 g of the as-prepared HCSs was first dispersed in 30 mL of copper nitrate solution with different concentration range from 0.03 to 0.24 M.
The reaction was terminated by adding 0.5 mL of copper reagent and boiled for 10 min.
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The injection solution consisted of 0.5 mmol (49.4 mg) of copper chloride and 10 mL of OA mixed together within a glovebox at room temperature.
To this mixture 1 mL of alkaline copper solution was added and kept at room temperature.
From the dilutions, 0.2 mL was transferred to different test tubes, and 2 mL of alkaline copper sulfate reagent (analytical reagent) was added before proper mixing.
200 mg of activated silicic acid was added and left aside for 30 min. The tubes were centrifuged and 3 ml of the copper layer was transferred to another tube containing 0.5 ml of diphenyl carbazide and mixed carefully.
Reaction was stopped by adding 1 ml of alkaline copper tartrate solution and incubated in boiling water bath for 10 min and cooled, then added arsenomolybdate solution for color stabilization.
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