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Briefly, 1 ml of complete medium was incubated with PRP-SF bioscaffolds for 24 hours.
Then the growth medium was removed from cells and replaced with 3 mL of complete growth medium.
Next, 1.5 ml of complete culture medium was pipetted into the basal chamber of the transwell inserts.
Cells were incubated for 10 min at room temperature and then transferred to a culture flask containing 10 ml of complete RPMI medium.
Briefly, complete medium was removed from bioscaffolds cultures and 1 ml of complete medium with 10% CCK-8 reagent was added incubating them for 4 hours at 37 °C in a 5% incubator.
The cells were washed 3 times and resuspended in 2 mL of complete medium.
After 4 h incubation at 37°C, the serum-free DMEM was replaced by 1 ml of complete DMEM.
Detached cells were dispersed in 4 mL of complete growth medium and gently pipette out of the well.
These C. albicans cells were washed with complete culture medium, counted with a hemocytometer and diluted to 5.0 × 106 cell/ml in 50 ml of complete culture medium.
The best clones resulting high intensity of bioluminescence were washed with hanks, trypsinized, centrifuged and resuspended in 1 ml of complete RPMI 1640.
Cells were then cultured in 10 ml of complete DMEM.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com