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Histones were extracted by resuspending the pellet in 10 ml of cold 0.4 N H2SO4.
Supernatants were removed, and pellets were dispersed in 1 ml of cold PBS.
The beads were centrifuged down at 10,000 × g for 1 min and washed three times with 1 ml of cold IP buffer.
The product was collected as a white precipitate and washed twice with 5 ml of cold DI water to yield >95%α-hydroxyglycine29α-hydroxyglycine29
Six grams of graphite powder was put into 120 mL of cold concentrated H2SO4 °C °C).
This DCM solution was added dropwise to 30 mL of cold hexane to reprecipitate the compound.
The mixture was added slowly to 20 mL of cold hexane, and a white-yellow precipitate was formed.
The mixture was cooled down in an ice-water bath and was added to 3 mL of cold deionized water.
1. Method #1: Pour 1 cup (250 ml) of cold water in pressure cooker.
10 ml of cold HF was added.
After incubation, cells were washed with 1 ml of cold PBS.
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