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Take 5 ml of clear filtrate.
Once 500 mL of clear fluid was tolerated, they received a regular diet.
Over 20 ml of clear ascitic fluid was immediately drained, and compression of the tense abdomen was released.
A 14-French silicone Foley catheter was inserted per urethra without any delay and 300 ml of clear urine was drained.
To make AgNPs, 5 mL of clear aqueous extract of H. asiatica was added to 10 mL of 10 mM AgNO3 (Spectrochem, Mumbai, India, AR Grade) and 85 mL of double distilled water so as to make the final concentration to 1 mM.
Photoluminescence (PL) and UV-visible (UV vis) absorbance spectra were obtained using a PerkinElmer LS50B fluorescence spectrometer (Waltham, MA, USA) and a Cary 50 UV-visible spectrophotometer (Agilent Technologies, Inc., Santa Clara, CA, USA), respectively, where 3 mL of clear aqueous solutions containing TiO2 nanoparticles were placed in quartz cuvette cells for the optical analysis.
Similar(31)
Nuclei and cellular debris were pelleted by centrifugation at 1000 rpm for 8 min. Then, 1 mL of cleared supernatant was mixed with 1 mL of 85% sucrose in TNEV and transferred to the bottom of a Beckman 14×95-mm 14×95-mmge tube.
1.5 ml of GSH bead slurry (GE Healthcare) was added to 50 ml of cleared lysate.
The HaloLink resin (1.0 ml) was added to 10 ml of cleared lysate.
Dissolution of paraffin was accomplished by the addition of 1 mL of clearing agent (Histochoice) and incubation at 65 °C for 30 min.
Antibody-coupled beads were washed and equilibrated with lysis buffer before incubating with 0.5 ml of cleared cell lysates for 16 hr at 4°C.
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