Sentence examples for ml of clarified from inspiring English sources

All strains were grown for 24 h in LB medium and 1.5 ml of clarified supernatant for rhamnolipid extraction was obtained from each culture by room temperature centrifugation (16,000× g).

Add 4 grams of ammonium sulfate for every 10 ml of clarified cell lysate and stir for 20 min at 4oC to dissolve the salt.

Polyallomer centrifuge tubes of 12 ml were filled with 1.5 ml of each iodixanol step and 3 ml of clarified homogenate in the top of tube.

Infected Sf9 cell culture supernatants were harvested at 50 to 60 h post infection (pi), and clarified by centrifugation at 2,400 rpm and 4°C for 10 min. Aliquots (11-ml) of clarified culture supernatant were subjected to ultracentrifugation at 28,000 rpm for 1 h at 4°C through a 1-ml sucrose cushion (20% sucrose, w:v in PBS) in Beckman SW41 rotor.

Cellular material was pelleted by centrifuging the supernatants for 1 min at 16,100×g and then 1 mL of each clarified supernatant was passed through 0.45 µm filters to further remove any cellular components.

After 24 h, cells were lysed in 0.5 ml of CLB, clarified by centrifugation, and then diluted to 2 5 mg/ml in CLB.

At room temperature, 1.0 mL of the clarified supernatant was loaded onto a methyl HIC column fast flow, pre-equilibrated with 2 M (NH4 2SO4.

About 0.5 mL of the clarified supernatant was transferred to a 10-mL glass tube with stopper that contained 2 mL of 2 M NaOH, 2.4 mL of glacial acetic acid and 350 μL of 0.5 M hydroxylamine.

Biomass was resuspended in 1/100 of the culture volume, homogenized in a French Press, and 1.2 ml of the clarified lysate was added to 4.05 mg HFB1 in 25 ml buffer (20 mM Tris HCl pH 8.0, 6.6 mM pyruvate, 30 μM PLP, and 0.1 mg/ml BSA).

One hundred millilitres of clarified supernatant was added to 300 ml of 100% ethanol (Molecular Grade, Sigma-Aldrich, St Louis, MO) and chilled at −20°C overnight.

The fermentations were conducted in 125-mL flasks containing 50 mL of fermentation medium (clarified sugar cane juice or molasses with approximately 14 17 g 100 mL−1 of total reducing sugars, pH 4.5 as supplied by a local fuel ethanol-producing unit, and sterilized at 120 °C for 20 min).