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After 24 hr, cells were washed twice with 1 ml of 30% PBS, one minute per wash. 1 ml of chloride salt vesiculation buffer (Del Piccolo et al., 2012) was added to each well, and the wells were incubated at 37°C, 5% CO2.
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Chloride-liberal fluids may cause renal dysfunction Large volumes (>3,200 ml) of chloride-liberal fluids infused in the first 24 hours after liver transplantation were associated with a higher risk of AKI.
After adjusting for covariates, infusion of greater than 3,200 ml of chloride-liberal fluids (HR 6.25, 95% CI 2.69, 14.5, P <0.001) and preoperative MELD score (HR 1.08, 95% CI 1.02, 1.15, P = 0.004) remained significant predictors for AKI.
After stepwise multivariate regression, infusion of greater than 3,200 ml of chloride-liberal fluids (HR 6.25, 95% CI 2.69, 14.5, P <0.000) and preoperative MELD score (HR 1.08, 95% CI 1.02, 1.15, P = 0.004) remained significant predictors for AKI.
The cells were harvested by centrifugation at 3000 g for 5 min at 4°C washed twice with sterile 0.9% (w/v) sodium chloride solution and resuspended in 2.5 mL of sodium chloride solution 0.5% (w/v).
Afterwards, 1 ml of barium chloride was added and mixed for another 3 min.
Animals were euthanized with a bolus infusion of 10 ml of potassium chloride 19.1% at the end of the experiment.
In short, dried nanoparticles (5 mg) were dissolved in 1 ml of methylene chloride under vigorous vortex.
Briefly described, 100 mg of BSA was dissolved in 1 ml of sodium chloride solution (10 mM).
3.6 GBq in 1.4 mL of 90Y chloride solution (PerkinElmer, Waltham, MA) was shipped to each institution.
A volume of 750 mL of cadmium chloride solution of various concentrations (5, 10, 15, and 30 mg/L) were prepared.
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