Sentence examples for ml of chilled from inspiring English sources

Then, 3.7 ml of chilled 50 wt% glyoxylic acid in H2O (0.52 eq, 2.5 g) was added and the reaction was stirred at 0 °C for 45 min.

and transcardially perfused with 30 ml of phosphate buffer saline (PBS), 0.1 mol/l, pH 7.4, followed by 60 ml of chilled paraformaldehyde (4%) in PBS.

Mature and well-exposed leaves from plant (0.5 g fresh weight) were homogenized in a mortar and pestle using 10 ml of chilled 80% acetone.

The resulting reaction mixture was refluxed for half an hour and cooled, and 100 mL of chilled, distilled water was added.

Each 25 g sample was homogenized in 50 mL of chilled extraction buffer containing 50 mM Hepes (pH 7.5), 500 mM sucrose, 1 mM DTT, 5 mM ascorbate and 1% polyvinylpolypyrrolidone (PVPP).

The supernatant was poured off and viscous pellet washed three additional times with 5 ml of chilled (4°C) sterile isotonic phosphate-buffered saline solution (PBS) solution, adjusted to pH = 7.4.

The pellet was resuspended in 1 ml (25 ml culture) or 3 ml (100 ml culture) of chilled 50 mM Tris, pH 7.5, and aliquoted (1 ml/tube) into 1.5-ml Protein Lo-Bind tubes (Eppendorf North America, Westbury, NY).

Cells were centrifuged at 300 g for 5 min and resuspended in 1 mL of 90% chilled methanol.

The buffer solution with chymotrypsin was chilled at 4 °C, and 10 μL of peptide solution was added to 1 mL of a chilled buffer solution to initiate the reaction.

The air-dried and powdered sample (2 g) was extracted with 50 mL of 80% chilled acetone at 4°C for 1 h and then the mixture was filtered with vacuum pump.

Each of the finely powdered samples (5 g) was extracted with 125 mL of 80% chilled acetone at 4°C for 1 h and then the mixture was filtered with vacuum.