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The culture was kept in a 250 mL Erlenmeyer flask containing 50 mL of broth.
Firstly, one loop of P. aeruginosa was added to 25 mL of broth culture medium to prepare bacterial suspension.
All strains were cultivated at 30 °C, 180 rpm in 200 mL of broth in a 1-L flask.
Rhizobium inoculant was prepared by mixing 30 g of sterilised decomposed filter-mud with 15 ml of broth cultures of the appropriate Rhizobium strain in polyethylene bags.
Two mL of broth was extracted by a graduated syringe and injected to a 5-mL centrifuge tube for pH measurement.
Rhizobium inoculant was prepared by mixing 30 g of sterilized decomposed filter-mud with 15 ml of broth culture containing HUPvR-16 strain in polyethylene bags.
Similar(20)
Likewise, sterile borosilicate glass test tubes (Corning) were incubated with 1 ml of brucella broth, brucella broth containing chicken juice, or 100% chicken juice.
Samples were agitated for 1 minute in 250 mL.1% peptone broth; then 30 mL of inoculated broth was mixed with 30 mL of 2X Baird Parker broth (BD) and incubated at 37°C for 18 24 h.
Before use, the bacterial strain was incubated in 50 mL of MRS broth (DifcoTM Lactobacilli MRS Broth, Becton Dickinson, Franklin Lakes, NJ, USA) at 37°C overnight.
Before use, the bacterial strain was incubated in 50 mL of MRS broth (Difco TM Lactobacilli MRS Broth, Becton Dickinson, Franklin Lakes, NJ) at 37 °C overnight.
One ml of this broth was added to Tryptic Soy Broth (TSB) supplemented with cefoxitin (3.5 mg/l) and aztreonam (75 mg/l) and incubated overnight at 37°C.
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