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Because there was a range of injected activities for clinical studies, the physical unit chosen for absolute quantitation was the percent uptake of injected activity per mL of brain tissue.
Dissimilarly, the volume (1 mL) of brain homogenized samples was 10-fold compared to plasma samples (0.1 mL).
TCBF was also expressed in mL blood per 100 mL of brain parenchyma per min (mL/100 mL/min).
One of the tubes contained 0.5 mL of brain heart infusion (BHI) with 15% glycerol as a cryoprotective agent for microbiota analysis.
7. Homogenise : Pour 15 mL of brain extract from the T75 into the homogeniser.
Swabs were incubated directly into 10 mL of Brain Heart Infusion at the site of collection.
Similar(32)
The E. coli strains were grown overnight in 45 mL of Brain-Heart-Infusion (BHI) broth.
For DNA preparations, strains were grown overnight in 45 mL of Brain-Heart-Infusion (BHI) broth at 37°C.
Briefly, an aliquot (200 μL) of Kokuria rhizophila was inoculated in 3 mL of brain-heart infusion (BHI) broth and incubated at 35°C for 24 h.
For C. difficile, cloths were immersed in 30 ml of brain-heart infusion broth supplemented with 0.1% sodium taurocholate and incubated anaerobically at 37°C for 5 days.
Tissue specimens were homogenized in 3 ml of brain-heart-infusion broth for 1 min and the homogenate was inoculated in aliquots of 0.5 ml.
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