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The cell suspension was poured dropwise into 150 ml of boiling 5% sodium dodecyl sulfate (SDS) solution and boiled for another 30 min.
After a final centrifugation (2,000 g, for 5 min, at 4 °C), 6 mL of boiling 50 mM EDTA was added to the CZ pellet and the homogenate was boiled for 7 min at 100 °C16.
The pellet was dissolved in 10 ml of boiling chloroform, which was subsequently evaporated and recycled.
Aqueous extracts were prepared using 5 g of dried powder in 100 ml of boiling water for 2 h.
The target was dissolved in 4 5 mL of boiling 8.0 M HCl for 15 20 min.
Fifteen gram of instant green tea powder was socked in 100 ml of boiling distilled water for 5 min.
The plant extract sample was then added into 125 ml of boiling deionized water, and left to boil for 3 min.
The solution was heated in a covered beaker for 3 h by stirring constantly and filtered; the residue was then washed with 40 mL of boiling distilled water.
An amount of 1 g of plant powder was prepared for infusion in 200 mL of boiling ultrapure water for approximately 10 min.
Strontium chromate was produced by adding an aqueous solution of strontium nitrate [1.1 g (0.005 mol) of Sr(NO3 2 dissolved in 25 ml of boiling H2O] to an aqueous solution prepared by dissolving 0.75 g (0.0025 mol) of K2Cr2O7 in 25 ml of boiling H2O and then adding Na2CO3 until a neutral pH was reached.
In this method, 0.5 g of each type of leaf powder was dissolved in 100 ml of boiling (distilled) water and subsequently allowed to infuse for 15 min without additional heating.
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