Sentence examples for ml of block from inspiring English sources

8 ml avidin (Vector labs Avidin/Biotin Blocking System) was added to a total of 50 ml of block buffer.

The nitrocellulose membrane was blocked in 20 ml of Block Buffer for 1 hour at room temperature, followed by washing twice for 5 minutes with 50 ml of PBST (1×PBS, 0.1% Tween-20).

The membranes were incubated for 2 h with the protein-A or anti-IgG conjugates in 5-10 ml of blocking buffer following by washing with TBS-TT.

Membranes were incubated for 1 hr at 37°C with gentle agitation in primary antibody (F1-40 or a rabbit polyclonal to Clostridium botulinum A toxoid # 20641 [Abcam Inc., Cambridge, MA]) diluted 5 µg/mL in 10 mL of blocking buffer.

The cells were washed with 1 ml of blocking buffer, mixed gently, and pelleted at 500×g for 5 min. The cells were suspended in 100 µl Cy5-conjugated Donkey-anti-chicken IgY antibody, diluted 1∶4000 in blocking buffer, incubated on ice for 1 hr, and washed as above.

Unbound antibodies were removed through three further washes with 1 ml of blocking solution.

The membranes were probed with primary antibodies (1 500 1000) in 10 ml of blocking buffer overnight at 4 °C.

Proteins were transferred from within the gel onto a PVDF membrane and blocked with 50 ml of blocking buffer (PBS, 0.1% Tween 20 and 5% Bovine serumalbumin).

After discarding the blocking solution, one ml of blocking solution containing about 10 phage particle was added to the cells and incubated for 1 h at 37°C.

Human cytokine array membranes were incubated for 30 min in 2 ml of blocking buffer and afterwards for 2 hours in 2 ml of sample supernatant at 20°C.

Adsorbed plasma was adjusted to a 1 10,000 dilution in 10 mL of blocking buffer and incubated with membranes overnight in 50-mL conical tubes at 4°C with rocking.