Sentence examples for ml of assay from inspiring English sources

A total volume of 3.0 mL of assay mixture was reached by adding 0.05 mL of enzyme extract (0.05 mL of phosphate buffer saline (PBS) for the blank test).

For MDH activity, 3.17 mL of assay mixture contained 94.6 mM phosphate buffer (pH 6.7), 0.2 mM β-NADH-Na2 salt, 0.5 mM oxalacetic acid, and 1.67 mM MgCl2.

From the samples, 10 µl was taken and diluted in 4 ml of assay buffer.

The medium of the HEp-2 cells was replaced with the premix to allow infection for 2 h, after which 0.1 ml of assay medium was added.

The lipids were then reconstituted in 1 ml of assay buffer (100 mM MOPS-KOH, pH 7.5) to a final concentration of 20 mg/ml by vortexing for 15 min to form multilamellar vesicles.

The bead pellet was gently washed with 12 15 mL of assay buffer, centrifuged as described previously, resuspended (45 mL assay buffer, 5 mL 1 mM biotin), and incubated for 1.5 h with gentle agitation.

5 ml of molten Assay Top Agar (identical to assay agar, but containing 0.75% agar, 0.4% glucose, 0.02% casamino acids and 300 ng/ml doxycycline hyclate) at 40°C is mixed with a 50 µl thawed aliquot (∼5×107 cells per plate) of each strain of an assay pair, poured uniformly over the surface of a pre-warmed isolation plate, and allowed to solidify.

The activities were assayed in a 1 mL volume of assay buffer [25 mM Hepes buffer (pH 7.3) containing 0.12 M NaCl, 3 mM KCl, 1 mM MgCl2, 0.25 mM FAD, and 80 μM electron acceptor].

ATPase activity was determined by adding 50 μL aliquots from the assay described above to 1 mL of ATPase assay buffer.

Then, cells were washed two times with PBS and lysed in 0.4 mL of radioimmunoprecipitation assay buffer (RIPA buffer).

A 100 μL of lipase solution was mixed with 1.9 mL of the assay mixture and incubated at 37°C for 10 min. The final absorbance of the mixture was measured at 410 nm by a Jenway 6505 UV/Vis spectrophotometer.