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Esophageal infusion of 10 mL of acid resulted in a 1.5-fold increase in total lung resistance compared to nearly a fivefold increase when 50 L of acid was instilled into the trachea.
99.2 ± 2.5% phosphorus removal from the ash was achieved under the following conditions: 60 °C, 13.9 wt.% of H2SO4 in the leaching solution, 4.80 (ml of acid solution)(g of ash)− 1, 0.0370 ± 0.0370 mm particle size.
This step was performed with ~2-5 g of resin mixed with ~20 mL of acid four times.
One milliliter of filtrate was taken and mixed with 1 mL of acid ninhydrin and 1 mL of glacial acetic acid in a test tube.
For each gram of calcinated sample, 4 ml of acid mix was added in order to eliminate impurities (calcium, potassium, magnesium, manganese, iron, boron and phosphorous).
To a ground sample (2 g), 9 ml of acid acetone (90% acetone, 8% deionized water, and 2% HCl, v/v/w), were added.
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The chromogenic reagent was prepared just before use by adding 5 mg of thiosemicarbazide to 50 mL of diacetyl monoxime solution (500 mg of diacetyl monoxime dissolved in 100 mL of distilled water) and mixing in 100 mL of acid-ferric solution.
After removing the membrane bound 125I-Ang(1 12), the myocytes were scraped using a cell lifter in 1 mL of acid-ethanol (0.1N HCl + 80% ethanol).
At the end of exposure time, the washed cells were collected in 1 mL of acid-ethanol (0.1N HCl+80% ethanol) and stored at −80°C to analyze the cellular uptake of 125I-Ang(1 12) by HPLC.
For the optimization study, the biohydrogen production was measured in 125-mL serum bottles containing 50 mL of acid-hydrolyzed SCB derived under different operating conditions.
One hundred gram of leaflets were ground in 100 mL of acid-methanol (4% v/v HCl) and filtered through Whatman filter paper number 1 (Whatman Ltd., Maidstone, UK).
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