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Water was sampled (5 ml in triplicate) 0.5, 1.5 and 2.5 hours after spiking.
Samples (5 ml, in triplicate) were taken through the septum using a 10 ml airtight glass syringe, after which they were injected into 12 ml Exetainers (Labco, high Wycombe, UK).
Newly formed pupae were collected (~40 ml) in triplicate before irradiation.
All samples were diluted to a total volume of 5 mL in triplicate.
Different dilutions of extracts (1 ml in triplicate) were added to 2 ml of DPPH (Sigma).
Cells were collected (1 ml in triplicate) and centrifuged (10,625 × g, 10 min, 4 °C).
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Intra-assay precision was assessed by analyzing varying concentrations of MLT (40, 60 and 80 ng ml-1) in triplicate in one assay batch.
SSF was performed at 200 mL, 10 mL and 1 mL scales in triplicate.
The medium was replaced with increasing MNP concentrations (1.56 to 25 μg mL-1) (in triplicates).
THP-1 and Jurkat cells, cultured as described above, were seeded on 96-well plates (at a concentration of 5 × 10 THP-1 cells per 0.1 ml well−1 and 2.5 × 10 Jurkat cells per 0.1 ml well−1) in triplicate, and various concentrations of MXF, VP-16 and their combination were added.
During the exponential growth phase, liquid culture (500 µL) was transferred to 1.5 mL microtubes in triplicate and centrifuged at 4 °C for 10 min.
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