Sentence examples for ml in eppendorf from inspiring English sources

All reactions were carried out in a buffer containing 50 mM Tris∙HCl pH = 8, 100 mM KCl, 15 mM MgCl2, 2 mM DTT and 20% (v/v) glycerol in a total volume of 0.1 1 ml in Eppendorf tubes.

Aliquot 100 ml in eppendorf tube.

After a brief sonication on ice, cell lysates (1 mL in eppendorf tubes) were rotated gently at 4 °C for 30 min followed by centrifugation at 20000 g at 4 °C for 20 min. Supernatants were transferred to tubes precooled on ice.

For crystal cell visualization by heating, ten third instar larvae were heated in 0.5 ml PBS in eppendorf tubes for 30 min at 67°C.

Aldehydes and amines (0.075 mmol each) were dissolved in anhydrous THF (0.5 mL) in an Eppendorf vial, and the solution was transferred to a dry reaction vial containing pre-activated 4 Å MS (300 mg) under N2.

After 24 h, 50% (w/v) glycerol was added, and aliquots of 1 mL were stored in Eppendorf tubes at −80°C until the experiments were conducted.

The 1 mL fractions were captured in Eppendorf tubes that contained 200 μL of 1 M Tris pH 8.8 to immediately neutralize the antibodies.

Tissue was placed in chilled TRIZOL (150 ml) in 1.5 ml eppendorfs tubes according to the manufacturers specifications.

After the washes the cells were resuspended in a 1.5 ml Eppendorf tube in 1 ml of ice-cold PBS-G containing 1∶100 SSEA3 antibody (Millipore, mab4303) and incubated for 45 minutes in the dark at 4°C with gentle rocking.

The invertebrates were collected and depending on their size placed individually in either 2 ml Eppendorf or in 50 ml Falcon tubes.

The PCR was performed in 0.2 ml eppendorf tubes in a thermocycler PT C-200 (MJ Research , Inc.