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For the coarse and fine PM fractions, PUF strips were transferred to sterile 50-cc polypropylene tubes and submerged in 35 mL of analytical-grade methanol.
Standard stock solution of SF was added to screened drug-free rat plasma to obtain concentration levels of 0.1, 0.2, 1.0, 5.0, 10.0, 40.0, 60.0, 80.0, and 100.0 ng/mL for analytical standards and 0.1, 0.3, 50.0, 75.0 ng/mL for quality control (QC) standards and stored in a −30° C freezer until analysis.
Gradients of B in 30 min were run at flow rates of 0.8 mL/min and 4 mL/min for analytical and preparatory procedures, respectively.
The flow rate was 1 mL/min for analytical HPLC and 2.5 mL/min for semipreparative HPLC, with the mobile phase of solvent A (0.1% TFA in water) and solvent B (0.1% TFA in acetonitrile).
For analytical size-exclusion chromatography a Superose 12 10/300 column (GE Healthcare) was equilibrated with buffer C at 0.5 ml/min and calibrated with protein standards of known Stokes radius.
Qualitative research aims for analytical generalization.
For analytical measurements, conduct interlaboratory comparison trials.
Grab samples (2250 ml) from each site were collected -two litres for analysis of antibiotic residues and 250 ml for analysis of antibacterial susceptibility - and were stored in an icebox at 4°C until they reached the analytical laboratories.
Fatty acid analytical procedure - The material was treated with 4 N-HCl in methanol (1 ml) and n-hexane (1 ml) for 2 h at 50°C.
Even something called crazy milk (300 ml for $12).
Aliquot 5.65 ml portions in analytical snap caps vials.
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