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About 10 g of biofilm from roots, water, gravel, and sludge samples from each FTW was incubated aerobically at 30 °C in 180 mL containers containing phosphate buffer at pH 8.0 and ammonium sulphate solution on a rotary shaker.
For now, the 500 ml containers, which retail for $8.99, are available on Amazon.
After cleaning the particles, samples intended for XPS analysis were placed in glass vials, flushed with N2, crimped, and transported in vacuum containers capable of maintaining vacuum of 14.7" Hg for over 24h, (Desi-Vac 700 mL containers, Cole palmer, USA).
Crayfish were housed individually in 946 ml containers placed in a 180×90 cm flow-through water table.
The three samples were collected in labeled 250 ml containers and preserved with 20 ml of 37% formalin.
After 12 hours of food deprivation, bumble bees were cooled in 750 ml containers until immobilized and secured in metal holders.
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Optimum popping conditions were found to be single layer grain mass in 250 mL container with popping percentage of 75.90%, expansion ratio of 6.03, beginning of popping at 15.86 s and threshold energy of 15.93 kJ.
Fifty grams of P. australis free soil was moistened with 30 mL litter leachate at four different concentrations (0, 2.5, 5.0, and 10.0%) in 250 mL container, and incubated for 24 h.
Our group's previous 177Lu quantification studies [13], based on the 113 keV photopeak using low-energy high-resolution (LEHR) collimator and the analytical photon distribution interpolated (APDI) scatter correction method [14, 15], resulted in 2% accuracy error for a 70 ml container scanned in air and in water (without background activity).
The net was rinsed and poured into a 125 ml container with 10 12 drops of Lugol's solution.
After three hours all eggs were transferred to a 750 ml container and held at a constant temperature of 20°C until hatching.
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