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Y-ML checked the study search and carried out statistical analysis.
From that point, cells were collected in 500 ml fractions, checked under the microscope, and centrifuged in 50 ml sterile polypropylene tubes.
Elution fractions (2 mL) were checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 5 12% Tris glycine gels (Laemmli 1970).
The reviewers (MM, ML) cross checked data and resolved any differences through discussion.
The frequent insertion of 5 to 10 ml air checked for kinking or misplacement.
Samples of each culture were taken before induction and after expression to prepare plasmid DNA (4 ml samples) and check protein expression (1 ml samples).
Fractions (0.2 mL) were collected and checked for rRNA by agarose gel electrophoresis.
The fractions (150 drops or about 5 ml) were collected and checked by SDS-PAGE (Coomassie Blue staining).
The trees from ML phylogenetic reconstructions were checked for consistency with alternative Bayesian phylogenetic reconstruction estimated with MrBayes [ 47].
Fractions of 1 ml were collected and checked for the presence of caveolin-1 and caveolin-2 by Western analysis using antibodies from Biosciences (San Jose, CA, USA).
Controls were instilled with saline containing gelatine (0.5 g/100 mL) or to check particle effects with suspensions of hematite (Fe2O3).
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