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Aqueous stock solution was prepared as 0.002 g/10 ml according to the manufacturer instructions.
The study of SSF was carried out in Erlenmeyer flasks (500 ml) according to the method described by Bhanu Prakash et al. (2008).
In paediatric patients, the volume of oral contrast is adjusted (between 300-1,000 ml) according to the weight of the patient.
The amount of bupivacain was varying between 2.5 3.0 ml according to the subject's height.
Drugs were administered intraperitoneally (IP) in a volume of 0.1±0.02 mL (according to body weight) for feeding tests.
The volume of water samples used for sedimentation varied between 50 and 256 ml, according to the overall biomass of phytoplankton as shown by fluorometry.
Similar(47)
Infectious virus titers were determined in Vero cells by calculating the 50% tissue culture infectious doses per ml (TCID50/ml) according to Reed and Muench [46].
Results were considered positive if the antibody titers were ≥250 International Units per mL (IU/mL), according to the manufacturer's guidelines.
Absorbance was read in a microplate reader at 450 nm and results were expressed in arbitrary units per milliliter (U/mL) according to the standards provided.
A training set of 25 standard mixture containing both drugs was prepared in the concentration range of 2 20 μg/ml according to mixture design.
Each sample was designated negative (0 ng/mL) or positive (>0 ng/mL) according to the LC/MSMS results.
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