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Following digestion with NcoI and BamHI, the library DNA was ligated into pET28a, the ligation mixtures were transformed into E. coli BL21, and transformants were screened as described above.
Reaction mixtures were transformed into E. coli strain DH5α as described above except transformants were selected on LB medium supplemented with kanamycin (50 μg/ml).
The ligation mixtures were transformed into MH13335, which contains pKM78, with P lacUV5- mor.
The ligated mixtures were transformed into Escherichia coli DH5α competent cells.
The purified mutant library plasmid mixtures were transformed into electrocompetent JA300A.
Then the ligation mixtures were transformed into Escherichia coli DH5α competent cells.
Similar(48)
All ligation mixtures was transformed to ElectroMAX E.coli DH10b competent cells (Life technologies).
Aliquots of the ligation mixture were transformed into competent E. coli TOP10 cells.
Both pUC19 (lac+) and pYD1 (lac−) were shown to have the same efficacy of transformation and the mixture was transformed into E. coli, followed by selection on LB Agar containing ampicillin and X-gal.
The ligation mixture was transformed into E. coli DH5α and the transformants were selected for chlorampheniol resistance.
The ligation mixture was transformed into E. coli XL1-Blue and the transformants were selected on low salt LB agar plates containing 25 μg/mL Zeocin.
More suggestions(15)
preparation were transformed
mixtures were cast
mixtures were sonicated
mixtures were maintained
mixtures were incubated
mixtures was transformed
mixtures were divided
mixtures were agitated
mixtures were diluted
mixtures were whipped
mixtures were blended
mixtures were injected
mixtures were designed
mixtures were allowed
mixtures were cooled
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