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Transformation mixtures were subsequently spread on LB agar containing erythromycin (5 μg ml-1).
The mixtures were subsequently cured at room temperature by a cycloaliphatic amine based curing agent to yield transparent epoxy silica hybrid coatings.
The mixtures were subsequently placed on carbon-coated grids and stained with 0.5% uranyl acetate and examined by electron microscopy.
The reaction mixtures were subsequently applied to a 1.8% agarose gel and the amplified products were stained with ethidium bromide.
PBDEs contained in these two mixtures were subsequently adopted as persistent organic pollutants (POPs) by the Stockholm Convention (Stockholm Convention Secretariat 2010).
The mixtures were subsequently transferred to polyvinyl Falcon plates pre-coated with peptide 131 151, and post-coated with bovine serum albumin in PBS-T (0.4% w/v).
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Aliquots (10 μL) of the reaction mixture were subsequently quenched by being mixed with 10 μL of formamide at time points ranging from 15 s to 60 min. Elongated DNA product in the quenched reactions was separated from the substrate DNA using 19% denaturing polyacrylamide gels.
The mixture was subsequently evaporated to near dryness.
The resulting mixture was subsequently heated to the temperature of 150 °C.
The reaction mixture was subsequently heated to 90 100°C and refluxed for 3 h.
The homogenized mixture was subsequently stirred to crosslink for 8 hrs.
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