Sentence examples for mixtures were spotted from inspiring English sources

Reaction mixtures were spotted on TLC Silica gel 60F254, 20 20 cm (EMD/Merck, Darmstadt, Germany) and developed with a mixture of n-Butanol/Acetic Acid/water (2 1.1 1, v/v/v).

Bacterial mixtures were spotted onto Hybond-C membranes, placed on Nutrient agar and incubated for 48 h at 28°C.

Bacterial mixtures were spotted onto Hybond-C membranes, placed on SB-agar and incubated for 48 h at 28°C.

Then, the reaction mixtures were spotted onto p81 paper for APP cytoplasmic peptide, or boiled in SDS sample buffer and subjected to SDS-PAGE for GST-fusion proteins.

After incubation, the reaction mixtures were spotted on Whatman P81 paper, washed, dried, and scintillation counted as described above.

After varying lengths of time, aliquots of the reaction mixtures were spotted on Whatman 3MM filter paper squares.

One μl of these mixtures was spotted onto each of the two PEI (Merck, Whitehouse Station, NJ, USA) plates.

Around 1.5×107 cells in the mid-log growth phase of the mixture were spotted in the centre of plates with HS agar -medium and exposed to the different irradiation sources.

Rab5 or Rab7 (1 µg) were loaded with [γ-32P]GTP by incubation with 10 µCi of [γ-32P]GTP in 100 µl of reaction buffer (50 mM HEPES pH 7.4, 50 mM NaCl, 0.1 mM DTT, 5 mM EDTA and 1 mg/ml BSA) for 10 min at 37°C. 10 mM MgCl2 was then added (to terminate the reaction) and incubated on ice for 10 min. Thereafter, 5 µl of each reaction mixture were spotted onto nitrocellulose membrane.

Aliquots of 2.5 μl of each reaction mixture were spotted onto a nylon filter (Wallac, Turku, Finland) and allowed to dry.

For the experiments under surface mating conditions, 8 μl of each resulting conjugation mixture were spotted into 96 well black microtiter plates (Thermo Electron Corporation) containing 300 μl LB agar.