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The NOD reaction mixtures were split into two fractions to which 4 μM FAD (final concentration) was added.
The mixtures were split into two fractions and thermolysin activity was inhibited in both the fractions with 0.4 mM (final concentration) phosphoramidon (Sigma-Aldrich, St . Louis MO).
D-loop formation and primer extension reactions were performed as described above, the resulting mixtures were split into two parts and electrophoretically separated in 0.8% (w/v) agarose gel in 1× TAE buffer, then lanes were excised from the gel.
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These 25 sample mixtures were splitted to 16 training mixtures (for building the models) and 9 validation mixtures (for measuring predictive power of the model).
The 150 μl reaction mixture were split into 3 tubes (50 μl each) and the PCR conditions were: 2 min at 95°C, 24 cycles of 10 s at 95°C, 30 s at 60°C, and 60 s at 72°C followed by a final extension for 1 min at 72°C.
In the first subunit the feed mixture is split into two fractions containing either a single component or a binary mixture.
The mixture was split into two 200-µl flat-cap transparent tubes.
After 5 min of preincubation, the reaction mixture was split in two.
Following this incubation, the reaction mixture was split into two halves and to one half a 10-fold excess of untagged duplex DNA (duplex 17) was added.
The reaction mixture was split into three aliquots.
The mixture is split into two tubes and the probe is precipitated by the addition of 95% ethanol (800 μl) to each tube.
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