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The enzymes in the mixtures were removed by ultrafiltration, and the filtrate was analyzed by LC-MS.
After incubation, aliquots of the mixtures were removed and centrifuged.
The transfection mixtures were removed at 6 h post transfection, and transfected cells were maintained for further processing.
Estimates from overlapping exposures (i.e. compounds appearing again in mixtures) were removed.
After 3 h at 37°C, mixtures were removed and replaced with 200 µl of complete media.
The data set was curated as previously described (Zhu et al. 2008): duplicate entries, entries with undefined molecular structure, inorganic, organometallic substances, and mixtures were removed.
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The supernatant containing the resulting peptide mixtures was removed and the gel pieces were re-extracted with acetonitrile.
The cell debris in the mixtures was removed using 0.45 μm low-protein-binding filters (Nalgene).
The air bubbles in the mixture were removed through degassing in a desiccator under a vacuum of 2 kPa for 1 h.
Unreacted 64CuCl2 and biotin-PEG-RGD2 in the reaction mixture were removed by centrifugation using a spin column (molecular weight (mw) cut-off 7 kDa).
After the tubes containing the first mixture were removed from the thermocycler and kept in a freezer at − 4 °C for 3 min, 6.5 µL of the second mixture was added to each tube.
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