Sentence examples for mixtures were pooled from inspiring English sources

Fractions 11-133) wereh were complex mixtures were pooled together and purified through flash chromatography (20 g, 230-400 mesh, 15 × 100 mm glass column C-690 Sepacore Buchi fitted with a precolumn of ID 15 mm) with EtOAc as solvent A and MeOH plus 2 drops ammonium hydroxide/100 mL as solvent B. The flow rate was set up to 2.5 mL/min.

After the PCR extension step, the amplicon mixtures were pooled in equal amounts.

PCR mixtures were pooled and purified by using the QIAquick PCR Purification Kit (QIAGEN) according to the manufacturer's instructions.

When more than 75% of recombinant clones were obtained, transformation mixtures were pooled together to constitute the library.

The two mixtures were pooled and 5 μL 4× hybridisation buffer (GE Healthcare, Amersham, UK) was added.

The lysis mixtures were pooled for each replicates and the qRT-PCR was performed as indicated above for the F2 families.

Four different RNA preparations from the same tissue mixture were pooled, visualized in a denaturing formaldehyde 1.2% agarose gel and quantified in a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE).

The resulting mixture was pooled and centrifuged at 20,000 g for 24 hrs.

To reduce variability among animals, a mixture of cells were pooled together from six and seven animals for the first and second phases of the study, respectively, to yield a representative mixture of chondrocytes.

All experiments were performed with two technical replicates and the RNA preparations were pooled mixtures of several rounds isolations for each sample, and are entirely independent from the ones used in RNA sequencing, hence the preparations itself contained multiple biological replicates.

The blood/PAXgene reagent mixture from donor tubes were pooled together.