The reactive mixtures were placed into Teflon moulds (Wilmington, DE, USA) where the OIS curing passed during 24 h at room temperature (T = 22°C ± 1°C).
The resulting mixtures were placed in a domestic microwave oven (900 W, 2.45 GHz, LG MO- MC-767 W/WS, LG Corp., Noida, India) and irradiated for 15 s.
Then, the transducers with deposited membrane mixtures were placed into glutaraldehyde saturated vapor for 15 to 25 min; afterwards, they were dried in the air at room temperature for 15 min and washed in the buffer solution from excess unbound proteins, zeolites, and GA.
The reaction mixtures were placed on TLC plates and developed twice in the following solvent systems: (1) nitromethane/1-propanol/water (2 5 1.5, v/v/v) or (2) ethyl acetate/acetic acid/water (3:1:1, v/v/v) with gallic acid, fructose, and sucrose as the standard materials.
These mixtures were placed in a 37°C water bath for the desired number of days (2 or 3 days for glycation experiments or 3 days for anti-glycation experiments with tested compound additions), and then stored at 4°C for further use.
These mixtures were placed in a 37°C water bath for the desired number of days (3, 6, 10, or 14 days for glycation experiments or 3, 5, or 7 days for anti-glycation experiments with tested compound additions), and then were stored at 4°C for further use.
Then, each of the mixtures was placed on MA104 or Caco2 cells for 1 h at 37 °C in a 5%% CO2 atmosphere.
Small drops of this mixture were placed on Parafilm and the grids were floated on it for 5 minutes.
The components of the mixture were placed inside a beaker glass, with a capacity of 1,000 mL.
Each 700 μl of this mixture were placed on a column and centrifuged at 13000 rpm at RT for 15 sec.
The reaction mixture was placed on a shaker at 300 rpm and incubated at 37 °C for 10 min.
