Sentence examples for mixtures were next from inspiring English sources

The resulting PCR mixtures were next diluted at a 1 10 ratio with water, and 1 μl of each was used for the second round of PCR.

The ethanol in the mixture was next evaporated at room temperature until a precipitate appeared.

The reaction mixture was next evaporated to dryness under vacuum and the residue was purified by gel permeation chromatography (Sephadex LH-20, GE Healthcare, 260×15 mm, methanol).

The mixture was next centrifuged in a Beckman SW41ti rotor (Beckman Coulter) at 36 000 rpm for 3.5 h at 4 °C, and the absorbance at 254 nm was determined with an EM-1 UV Monitor (Bio-Rad).

Total cell protein lysate was incubated with amino endoperoxide (4′-alkyne) for 1 h to capture its potential intracellular targets, the mixture was next subjected to CLICK-chemistry reaction with biotin-conjugated azide as described by the manufacturer (Life Technologies), enabling the affinity pulldown of the molecular complexes of 4-Me and its interacting targets by streptavidin beads.

Among nonmetal agents, polychlorinated biphenyls, organophosphate and organochlorine pesticides, particulate matter, and mixtures were the next most frequently studied.

were added to each well, and the mixtures were agitated for the next 24 h, filtered and washed as previously described.

In the next step, the mixtures were shaken for 360 min or 24 h and filtered.

In the next step the mixtures were shaken for 2 or 24 h and filtered.

These competitor antibody mixtures were incubated at room temperature until the next blocking step was done.

Trifluoroethylene and its mixtures are useful as next generation refrigerants because of their lower global warming potential (GWP) than that of those ordinarily used.