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Aliquots of the S and I peptide mixtures were fractionated by reversed phase chromatography on a silica-C8 reversed phase column (Waters, Elstree, Hertfordshire, UK) using a solvent gradient of increasing acetonitrile concentration containing 0.1% TFA.
Reaction mixtures were fractionated on 7% polyacrylamide (PAA)/7 M Urea gels.
Following incubation, the mixtures were fractionated on gels and the oligonucleotides were quantitated to obtain a stability index for each oligonucleotide.
Reactions were quenched at appropriate time intervals with excess EDTA, and mixtures were fractionated on denaturing polyacrylamide urea gels and quantitated on a Fuji FLA 5100 scanner.
Cross-linked mixtures were fractionated without boiling by SDS PAGE using 10 20% Tricine gels (1.0 mm × 10 wells, Invitrogen) and silver stained using a Silver-Xpress silver staining kit (Invitrogen).
Similar(55)
If effects are detectable, the mixture is fractionated according to the physico-chemical properties of the components.
Then 100 µL of 20 mM ammonium acetate, with 35 µM bromophenol blue as internal reference, was added and 100 µL of the resulting mixture was fractionated on a Nucleosil C-18 300-5 HPLC column (Macherey & Nagel) using a solvent gradient (first solvent: 20 mM ammonium acetate, second solvent: 97% acetonitrile).
Eight hundred micrograms of the peptide mixture was fractionated on either a PolySAX LP or PolyWAX LP column (PolyLC; 4.6 mm inner diameter (i.d).
The mixture was fractionated on a silica gel column (60 × 5 cm) by eluting with a gradient of chloroform: methanol (9 1) to separate the bioactive compounds.
The serum glycopeptide mixture was fractionated by C-18 RP column (4.6 mm diameter × 150 mm length) using an offline ultimate 3000 HPLC (Dionex, Sunnyvale, CA).
The combined peptide mixture was fractionated by strong cation exchange (SCX) chromatography on a BioCAD workstation (Applied Biosystems), using a 4.6 mm × 20 cm polysulfoethyl aspartamide column (PolyLC Inc, Columbia, MD, USA).
More suggestions(15)
mixtures were cast
mixtures were annealed
mixtures were sonicated
mixtures were maintained
mixtures were considered
mixtures were incubated
mixtures were agitated
mixtures were diluted
mixtures were injected
mixtures were designed
mixtures were cooled
mixtures were allowed
mixtures were studied
mixtures were tested
mixtures were filtered
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