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The mixtures were directly subjected to 5% native PAGE containing 0.5× Tris-borate-EDTA (TBE) buffer.

Quenched digestion mixtures were directly desalted and analyzed by MALDI-TOF as indicated above.

IPA extracts of reaction mixtures were directly analyzed by LC/MS.

Thereafter, the mixtures were directly applied to the cultivated cells (ie, primary human skin culture) or mouse skins, respectively.

Extracted protein mixtures were directly reduced with dithiothreitol (DTT), alkylated with iodoacetamide, and subsequently digested with endoproteinase Lys-C and trypsin as previously described [ 88].

Similar(55)

From previous studies, we conclude that grass crude protein (CP) content in alfalfa-grass mixtures is directly proportional to the percent alfalfa in the mixed stand.

A wide range of oxygen and methane mixtures was directly processed without dilution or explosion at ambient temperature because the microscale plasma reactor removes excess heat generated by partial oxidation, thereby maintaining a reaction field at temperatures near room temperature.

The authors' recommendation that toxicity of chemical mixtures be directly assessed on a case-by-case basis (Tinwell and Ashby 2004) would provide a Band-Aid but not a cure to the dilemma of characterizing the hazards of chemical mixtures.

The reaction mixture was directly microfiltered after the addition of acidified water without previous settling.

The mixture was directly submitted to flash chromatography on silica gel with pentane EtOAc (3/1) as eluent affording product 9 (65% yield for (P,P -trans-L2; 90% yield for (M,M)-cis-L2; 85% yield for (P,P -trans-L2

That is, N,N′-bis trimethylsilyl -substituted m-pheN,N′-bis trimethylsilyl -substitutedthaloyl chloride iN,N′-bis trimethylsilyl -substitutedamide, and the resulting mixture was directly cast into films.

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