The concentrations of eight ILs in mixtures were set up by the uniform design.
The number of mixtures is set to 4. In this paper, we explored more effective features for the classification of neutral and stressed speech based on a physical model.
Two reaction mixtures were set up per transfection.
All reaction mixtures were set up in triplicate, and the average values were used for recording and calculation.
The PCR mixtures were set up using 10 μM XPDN-18 5'-CTGATCACTCGAGAGATC, 0.4 mM dNTPs, 10 × PCR buffer, 1.5 mM MgCl2 and 1U Taq DNA polymerase (Invitrogen).
For fluorescence polarization, mixtures were set up containing the indicated concentration of protein, 10 n m Lumio-Green-labelled peptide in a final volume of 30 μl.
For fluorescence polarization, mixtures were set up containing the indicated concentration of protein, 10 nM Lumio-Green-labelled peptide in a final volume of 30 μl.
Various combinations of the used primers are presented in Table 5.> For cox1 and ITS, 25 μL PCR mixtures were set up with 0.5 U Taq polymerase (Qiagen), 5 μL 10x buffer, 2 4 μL MgCl2 (depending on template and primers), 0.8 μL dNTPs, 0.5 μL from each primer (20 ng/μL).
Data from these dilution experiments were used for Figure 1, Figure 2, Figure 3, Figure 4, Figure 7, Figure 8, Table 2, Table 3, Table 4 and Table 5. (ii) In range experiments two different mixtures were set up to cover a wide range of signal intensity.
