Exact(3)
The powder materials were design to have three total powder contents of 450, 550 and 650 kg/m3, whereas the water-powder (OPC plus ISFC) material ratio for all mixtures was kept constant at 0.40.
For whole-cell recordings the α3 to β4 cDNA ratio in the mixtures was kept equal (1∶1), whereas for single-channel recordings we used extreme ratios of 1∶9 or 9∶1 of wild-type subunit cDNAs.
The mixtures was kept under darkness and stirred for 12 h at room temperature.
Similar(57)
The mixtures were kept in 25°C.
The mixtures were kept on ice for 60 min.
Therefore, the prepared mixtures were kept under minimum temperature fluctuations without direct sun light exposure.
The mixtures were kept at −80°C for 30 min and centrifuged at 14,000 x g.
Without heating or ultrasonication, the reaction mixtures were kept at room temperature for 48 h.
The DNA/mRNA mixtures were kept at −80°C until use.
Reaction mixtures were kept constantly stirred at 700 rpm.
Mixtures were kept in dark at ambient conditions for 2 h to complete the reaction.
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