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The other 13 samples contained mixtures of variant subpopulations.
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Overall, 24.4 % of the samples with heterozygous loci were found to contain mixtures of genotypes (n = 20), while the rest represented mixtures of variants of a single genotype (n = 62).
Literature review suggests that the gearbox vibration is a kind of nonlinear mixture of variant vibration sources and the blind source separation (BSS) is reported to be a promising technique for fault vibration analysis, but very limited work has addressed the nonlinear BSS approach for hybrid faults decoupling diagnosis.
Regardless of the particular mixture of variant numbers and their effect sizes, the overall polygenic risk would need to increase by approximately 4.8-fold per standard deviation in order to explain the observed FRR.
In addition, due to the high copy number of mtDNAs, a mutation in some of the mtDNA results in a mixture of variant mitochondrial genomes within a mitochondrion, cell, tissue, organ, or individual.
Interestingly, studies with the 1918 pandemic H1N1 virus describe it as circulating as a mixture of variants with either 225D or 225G [5], but the biological significance of this heterogeneity remains unknown.
In the present study of the isolate sequenced, which was a mixture of variants, we detected 17 positions distributed along the genome that were highly polymorphic (variable in more than 20% of the reads; Fig. S1a), all of which were silent or neutral nucleotide (nt) substitutions.
Each peak comprises a mixture of variants, the number of which is probably critical to the balance between antibody-mediated and density-dependent limitation of growth.
Using gold-standard data sets compiled from the emerging large-scale functional genomics and proteomics experiments, an increasingly wider range of reference genomes and a mixture of variants and parameters [ 10, 11], these 'genome-aware' sequence analysis methods and in particular gene cluster/fusion detection, have yielded an impressive level of performance and accuracy [ 12].
This Sanger based technique is capable of detecting mixtures of viral variants when each variant represents >15% of the viral population.
Separations of these complex mixtures using this new instrumentation demonstrate the potential of ultra-high voltage CE to identify the presence of previously unresolved components and to reduce analysis time for complex mixtures of antibody variants and drug conjugates.
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