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For competition EMSAs, unlabeled double-stranded oligonucleotide was added to reaction mixtures before the radiolabeled probe.
For competition experiments, unlabeled blunt-ended competitor oligonucleotides were added to the binding reaction mixtures before the addition of the radiolabeled oligonucleotide probes.
The OD400 values of the reaction mixtures before the addition of GlyPro- p-nitroanilide were subtracted from the obtained values to represent the real increase of OD400 values as a measurement of proteolytic activity.
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The company tested more than 1,200 different mixtures before finding the combination with the right material properties.
The modification includes an add-on device in order to purge the Hartmann tube with the gas of the hybrid mixture before the dust dispersion and ignition attempt.
The chosen shock strength ignites the gas mixture before the shock-focusing point, followed by a detonation wave, which propagates through the entire bubble gas.
The obtained results indicate that boiling of the organic mixture (before the magnetic fluid itself reaching boiling point) effectively enhances the heat transfer as well as boosting the flow to circulate in the closed loop by itself.
PSi NPs or Si NSs in colloidal suspension were introduced into the mixture before the gel formation stage in concentrations from zero to 0.34 mg/ml.
Ten microlitres of bovine serum albumin (BSA pH 6.8; 50 mg/mL) was added to the reaction mixture before the PD10 column purification.
To determine the bacterial count in the reaction mixture, 0.1 mL of the reaction mixture, before the addition of perchloric acid, was diluted by a factor of five with a sterilized solution of 0.9% NaCl.
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CEO of Professional Science Editing for Scientists @ prosciediting.com