After incubation, the mixtures were probed with an anti-transcription factor antibody, washed by centrifugation, detected with a fluorescent secondary antibody, and washed again.
On the basis of the newly developed apparatus and method, the effects of pressure, shear rate and temperature on the apparent viscosity of CO2-heavy oil mixture were probed.
In addition, the synergism observed in liquid unloading performance of formulations containing binary surfactants mixture was probed by studying the Critical Micelle Concentration (CMC) and molecular interaction in aqueous solutions.
In this situation, the unreactive strand could be paired (differently) in both structures, with the more reactive side of the helix remaining single-stranded in at least one structure within the mixture being probed.
The emulsion was transferred dropwise to an aqueous solution of sodium cholate (V = 20 ml, 12 mM) and the mixture was probe sonicated at 18 W for 3 min to form w/o/w emulsion.
The emulsion was transferred dropwise to an aqueous solution of sodium cholate (V = 20 ml, 12 mM) and the mixture was probe sonicated at an amplitude of 60 W for 3 min to form w/o/w emulsion.
The mixture was probe-sonicated for 10sec.
This single-phase mixture was probe sonicated for 30 sec at room temperature and then incubated overnight at 48 C.
Membranes were probed with a mixture containing anti-ORP150 antibody (1 : 100) or anti-HIF-1 α (1 : 500) in 5% dried milk in TBS-T, at 4°C, for 16 h.
Western blots were probed with a mixture (1 2000 dilution) of two monoclonal anti-CFTR antibodies, M3A7 and MM13-4 (CheMilliporeillipore, Darmstadt, Germany) recognizing distinct epitopes to increase sensitivity as CFTR has low expression levels in native tissues [ 27, 28].
