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The cell mixture were then triturated and plated on T75 flask (Corning, USA).
The interactions of glucose and sucrose mixture were then manipulated by CCD to identify the value of response positioned at the maximum removal of NO3 − N.
The mixture were then added into wells of Biolog Microplates PM13B, PM14A, PM15B, PM16A and PM18C containing substrates of various heavy metal salts.
The hardened mixture were then cut into uniform pellet size (approximately 2 mm × 2 mm × 2 mm) and stored in a refrigerator (4 °C) until further use (Lim et al. 2015, 2016, 2017).
The proteins in the mixture were then extracted several times with phenol-chloroform, and the genomic DNA was precipitated first with 2.5 volumes of 100% ethanol supplemented with 60 μl of 3 M sodium acetate buffer (pH 5.2), and then with 2.5 volumes of 70% ethanol for demineralization.
On half of the samples, an atmospheric plasma sprayed (APS) TiO2 bond coat was preliminarily deposited; suspensions of attrition-milled micron-sized glass powders, dispersed in a water + isopropanol mixture, were then sprayed onto both bare and bond-coated plates using five different process parameter sets.
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This mixture is then mixed with dilute hydrochloric acid.
The mixture was then shaken by hand.
The reaction mixture was then lyophilized.
The mixture was then incubated for 1 h at 120 °C.
This mixture was then passed over a QMA cartridge.
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