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The samples of reaction mixture were subjected to spectral analysis at resolution of 1 nm from 300 to 700 nm using dual beam UV vis spectrophotometer (Labomed, USA) to determine the surface plasmon peak characteristic of AgNps.
Aliquots of RT mixture were subjected to PCR cycles with appropriate primer sets.
The mixture were subjected to brief centrifugation, and absorbance of the supernatants fluids was measured at 540 nm.
To visualize the number of PCR products obtained, 2 μl of post reaction mixture were subjected to agarose gel electrophoresis.
As-prepared NPLs in the form of a crude reaction mixture were subjected to the cation exchange process, as depicted in the synthesis scheme in Figure 1.
For quantitative real-time reverse transcription-PCR (qRT PCR), each reaction mixture was diluted five-fold with DNase-/RNase-free water (Invitrogen), and 4 μl of each mixture were subjected to PCR.
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These were excised and the protein mixture was subjected to tryptic digestion and LC-MS/MS analysis.
The mixture was subjected to sonication (5 min) and centrifugation (3 min, 1800 rpm).
The hybrid mixture was subjected to ultrasonication for effective blending.
A given mixture is subjected to a temperature program consisting of symmetrical upward and downward temperature paths.
This mixture was subjected to semi-preparative HPLC purification.
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mixture were engineered
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mixture were calculated
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