Sentence examples for mixture were spotted from inspiring English sources

Around 1.5×107 cells in the mid-log growth phase of the mixture were spotted in the centre of plates with HS agar -medium and exposed to the different irradiation sources.

Rab5 or Rab7 (1 µg) were loaded with [γ-32P]GTP by incubation with 10 µCi of [γ-32P]GTP in 100 µl of reaction buffer (50 mM HEPES pH 7.4, 50 mM NaCl, 0.1 mM DTT, 5 mM EDTA and 1 mg/ml BSA) for 10 min at 37°C. 10 mM MgCl2 was then added (to terminate the reaction) and incubated on ice for 10 min. Thereafter, 5 µl of each reaction mixture were spotted onto nitrocellulose membrane.

Aliquots of 2.5 μl of each reaction mixture were spotted onto a nylon filter (Wallac, Turku, Finland) and allowed to dry.

For the experiments under surface mating conditions, 8 μl of each resulting conjugation mixture were spotted into 96 well black microtiter plates (Thermo Electron Corporation) containing 300 μl LB agar.

Samples were mixed 1 1 with a saturated solution of α-cyano-4-hydroxy cinnamic acid in 50% v/v ACN 0.1% v/v trifluroacetic acid and 2 μL of the resultant mixture were spotted onto a MALDI target.

At the end of incubation, the reaction mixture was spotted on 2 cm × 2 cm Whatman P81 phosphocellulose paper pieces.

tartrazine (TZ), carmoisine (CS), brilliant blue (BB), alizarin red S (AR), xylenolorange (XO) and bromo-cresol purple (BP) were mixed and 0.1 μL of the resultant mixture was spotted on the TLC plates.

The mixture was spotted on a MALDI target plate and allowed to dry at room temperature.

Then 20 µl from the cell mixture was spotted onto V8 agar medium and a total of 10 spots were added on each plate.

0.5 µl reaction mixture was spotted on the PEI cellulose plate and the plate was kept in saturated chromatographic chamber for developing the chromatogram.

After incubation at 70°C for 2 min, 2 µl of each mixture was spotted onto a TR plate, followed by incubation at 70°C for 16 h.