Sentence examples for mixture were split from inspiring English sources

The 150 μl reaction mixture were split into 3 tubes (50 μl each) and the PCR conditions were: 2 min at 95°C, 24 cycles of 10 s at 95°C, 30 s at 60°C, and 60 s at 72°C followed by a final extension for 1 min at 72°C.

In the first subunit the feed mixture is split into two fractions containing either a single component or a binary mixture.

The mixture was split into two 200-µl flat-cap transparent tubes.

After 5 min of preincubation, the reaction mixture was split in two.

Following this incubation, the reaction mixture was split into two halves and to one half a 10-fold excess of untagged duplex DNA (duplex 17) was added.

The reaction mixture was split into three aliquots.

The mixture is split into two tubes and the probe is precipitated by the addition of 95% ethanol (800 μl) to each tube.

The no-digest control mixture was split and subjected to two ddPCR duplexed assays of RASSF1/ RNaseP and RASSF1/ β-actin.

The beads were washed 3 times with 1% NT-2 buffer (1% Nonidet P-40, 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 2 mM EDTA) and the mixture was split in half.

The mixtures were incubated for 37 °C for 2 h, 60 °C for 2 h, then 65 °C for 20 min. The restriction enzyme digested mixture was split and subjected to three ddPCR duplexed assays of SRY/ TERT, RASSF1/ RNaseP, and RASSF1/ β-actin.

The 200 μl reaction mixture was split into 4 tubes (50 μl each); PCR conditions were 2 min at 95°C, fourteen cycles of 10 s at 95°C, 30 s at 52°C, and 120 s at 72°C followed by a final extension for 5 min at 72°C.