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The air bubbles in the mixture were removed through degassing in a desiccator under a vacuum of 2 kPa for 1 h.
Unreacted 64CuCl2 and biotin-PEG-RGD2 in the reaction mixture were removed by centrifugation using a spin column (molecular weight (mw) cut-off 7 kDa).
After the tubes containing the first mixture were removed from the thermocycler and kept in a freezer at − 4 °C for 3 min, 6.5 µL of the second mixture was added to each tube.
To halt the reaction, 50 μl aliquots of the mixture were removed from the reaction mixture and mixed with 450 μl of the stop solution, 1 M Tris-HCl (pH 7.0), at appropriate intervals, and the initial rate was estimated from the initial slope of the reaction time course.
At various times, 3 µl aliquots of the reaction mixture were removed for analysis, and BER products were observed as described previously [1], [4], [22].
Aliquots of the reaction mixture were removed at selected time points and treated as described previously (20, 24).
Similar(37)
Heat from the mixture is removed within the two regenerators of this tandem system.
In each group 20% of the cement content of the control mixture was removed, resulting in starting mixtures with 200, 240, 280, and 320 kg/m3 cement content.
After 5 h of incubation, bacmid Cellfectin mixture was removed and replaced with 2 ml of SF-900 II SFM 50 U ml−1 penicillin and 50 μg ml−1 streptomycin.
The remaining water from this mixture was removed azeotropically using acetonitrile.
The vaporized ethanol-water mixture is removed through a side draw and is then fed into the rectification column without condensation.
More suggestions(15)
solution were removed
mixture were dissolved
melt were removed
mixture were given
mixture were carried
mixture were tested
mixture were incubated
mixture were investigated
mixture were studied
mixture were measured
mixture were recorded
mixture were engineered
mixture were captured
mixture were found
mixture were calculated
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