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Furthermore, the separation efficiencies of copper foam on trichloromethane-water and diesel-water mixture were maintained above 96.9% and 90.8% after 10 cycles, respectively.
Tubes containing only the reaction mixture were maintained in the dark and served as control to reaction.
Then, 1 × 106 cells/reaction mixture were maintained in 0.5% paraformaldehyde/PBS for 10 min at 4°C, washed, resuspended in 150 μl of PBS containing 10% FBS, and blocked for 20 min at 4°C.
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The temperature of the mixture was maintained at 80 °C.
The mixture was maintained at 50 °C for 50 min.
A 1% isoflurane gas mixture was maintained during acquisition.
pH of the mixture was maintained constant as previously mentioned.
This mixture was maintained at 23°C for about 5 min.
The pH of the reaction mixture was maintained at 3.0.
The resulting mixture was maintained at room temperature under vigorous stirring condition for 2 h.
Finally, the reaction mixture was maintained at 72 °C for 5 mins before completion.
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