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For experiments with nanostructures, aliquots of the assembly mixture were gently homogenized prior to irradiation.
Briefly, 200 μl of chloroform was added, and the tubes with the lysis mixture were gently inverted for 5 min.
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Finally, the mixture is gently stirred together.
The mixture was gently stirred at 4 °C for 1 h.
Thirty minutes after mixing, the mixture was gently centrifuged to obtain the two-phase separations.
The mixture was gently agitated overnight at 4°C on a shaker.
The reaction mixture was gently refluxed and stirred for 24 h.
After cooling, 2.5 mL of 70%% HClO4 was added, and the mixture was gently boiled until dense white fumes appeared.
The above reaction mixture was gently mixed and incubated for 3 h with gentle and continuous vortexing.
Then equal volume of chloroform: isoamyl alcohol (24:1 v/v) was added and mixture was gently mixed for 10 min by inverting the tube.
The mixture was gently heated on a hot plate after a few boiling chips had been added and evaporated to about 20 mL.
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