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Approximately 300 ng of probe from this mixture were dissolved in 10.5 μl Hybrizol VII (Q-BIOgene) and 0.75 μg each of human and manatee Cot-1 DNA.
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An adequate amount of ethylene glycol was added into the solution after the mixture was dissolved.
For the wet cure, the same curing mixture is dissolved in water to create a brine in which the meat is soaked.
The lipid mixture was dissolved in the solution of chloroform and methanol (3:1).
After cooling the mixture was dissolved in chloroform and filtered off.
The reaction mixture was dissolved in diethyl ether and washed with water excessively.
The mixture was dissolved with 10 mL 0.10 mol/L H2SO4 solution and diluted to 25 mL with water.
After 5 days, the reaction mixture was dissolved in toluene and passed through an aluminum oxide column to remove the copper catalyst and then precipitated in n-hexane.
The above redox mixture was dissolved in 2 ml of deionized water and then it is taken into a muffle furnace at 500 °C.
Each mixture was dissolved in anhydrous pyridine (1 mL) and reacted with l-cysteine methyl ester hydrochloride (1.5 mg) stirred at 60 °C for 1.5 h.
After completion of the stress, the powder mixture was dissolved and diluted to 50 mL with mobile phase. 2 mL of this solution was further diluted to 10 mL with mobile phase.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com