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For SIM-GC-MS, 2 μl of derivative mixture were delivered by automatic injection to an HP-5890 gas chromatograph split-injection port (1 20) leading to a 0.2 mm × 25 m Chrompack CP-sil 19 CB (WCOT Fused Silica) capillary column.
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This mixture is delivered, via the common gas outlet, to a patient breathing circuit, usually a 'circle system'.
Then, the mixture was delivered into a microfluidic chip (Fig. 2A) and cultured for 1 hour to complete the gelation (Fig. 2B).
The gas mixture was delivered through the books mass flow controller at fixed flow rate.
After the dye-stimulus mixture was delivered, the mouse was euthanized by CO2 followed by cervical dislocation.
The gas mixture was delivered through a small anesthesia mask (David Kopf Instruments, Tujunga, CA) connected to a calibrated Fluotec 5 (Fluotec-Ohmeda, Tewksbury, MA, USA) vaporizer at a flow rate of 1 4 L/min oxygen.
The NO/N2 mixture is delivered from a cylinder.
The mixture was delivered subcutaneously, divided among three flank areas.
Animals were placed in a 70x70x50 cm chamber divided into separate cells where the chosen gas mixture was delivered at 3.5 l/min.
During image acquisition, a 2% isofluorane air mixture was delivered through a nose cone, and the body temperature was maintained at 37°C by a heated stage.
The cDNA mixture was delivered to the cells by Effectene® transfection (301425, Qiagen GmbH, Hilden, Germany) according to the manufacturer's guidelines.
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