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The DNA fragments amplified from this artificial mixture were cloned, and 75 random clones were sequenced.
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The three fragments were cloned into pET21a by Seamless Cloning.
Positive clones were cloned twice by limiting dilution.
This mixture of DNA fragments was cloned into a promoter probing vector (pVIK165).
The PCR mixture enriched for differentially expressed sequences was cloned using pGEMT (Promega) as mentioned above.
To validate whether the FLC-related gene (s1_contig20110 and s2_contig24845) exists in hickory or not, their primers was designed and the corresponding full-length CDS was cloned from a mixture of cDNAs of developing floral buds (Additional file 9: Figure S2).
d: The map1 PCR amplicon was a mixture of more than one sequence, and the product was cloned prior to sequencing.
It's cloned!
2. How is cloning accomplished?
A second option is cloning.
Sequences lacking electrophoretic mixtures were considered clones and those with electrophoretic mixtures were considered oligoclonal.
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