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To isolate the NPs, 50-mL aliquots of the NPs mixture were centrifuged at ~2,500 RPM for 5 min. The supernatant was decanted to remove excess PEG and reaction byproducts.
After the reaction completed, three kinds of bacteria mixture were centrifuged and washed with PBS three times and then resuspended in 1 mL of 0.1 M PBS (pH 7.4).
After the incubation, the contents (rumen fluid, toxin and methanol mixture) were centrifuged for 10 min at 10,000×g (Eppendorf centrifuge).
To assess the completion of carboxyl methylation, aliquots of the reaction mixture were centrifuged at 20000 g at 4 °C for 20 min to remove the Sf9 membranes, and the supernatants were injected onto the reverse-phase HPLC system.
To assess the completion of the cleavage reaction, aliquots of the reaction mixture were centrifuged at 20000 g at 4 °C for 20 min to remove the Sf9 membranes, and the supernatants were injected onto the reverse-phase HPLC system as described above.
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This mixture was centrifuged and the supernatant was removed.
The mixture was centrifuged for 10 min at 3000 rpm.
Then, the mixture was centrifuged at 17,000g for 30 min.
The mixture was centrifuged for 30 min at 4000g.
The mixture was centrifuged at 10,000×g for 30 min.
The mixture was centrifuged at 13,000 × g for 45 min.
More suggestions(15)
mixture were carried
mixture were incubated
mixture were tested
mixture were investigated
mixture were studied
mixture were observed
mixture were recorded
mixture were measured
mixture were engineered
mixture were captured
mixture were calculated
mixture were found
mixture were transferred
mixture were formed
mixture were taken
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CEO of Professional Science Editing for Scientists @ prosciediting.com