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At each time point, 10 μl reaction mixture were added to SDS loading dye to stop the reaction.
After preparation of the mixture of the siRNA duplex solution and transfection reagent, the siRNA transfection medium and the siRNA transfection reagent mixture were added to the cells.
100 µl of a mixture of alpha cypermethrin and lambda-cyhalothrin solutions (internal standards, Sigma-Aldrich) and 900 µl of a 90/10 isooctane/toluene mixture were added (Sigma Aldrich).
After cooling, 1 ml distilled water and 5 ml of n-butanol-pyridine (15:1; v/v) mixture were added.
10 μl of antibody mixture were added to 50 μl of blood and incubated for 30 min at room temperature.
To this mixture were added aliquots of 10 pmol D5-2-arachidonlyglycerol (2-AG) and 5 pmol D8-arachidonlyethanolamine (AEA).
Similar(19)
Breading for the crab cake mixture was added with a light hand.
After denaturation (80 °C for 7 minutes), the probe mixture was added to the denatured chromosome.
The heat is turned down completely, and the mixture is added to the pan.
Finally, the remaining mixture was added.
The reaction mixture was added into water, and then extracted by EtOAc for three times.
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